Preparing Drug Treatments in the Microfluidics Chamber
Preparing drug treatments in the microfluidics chamber is straightforward. Prepare a drug treatment at a concentration of 1.25 times the final concentration. Pipette this reagent into the top left well of the microfluidics chamber. Make sure to avoid bubbles while adding the reagent. Next, pipette the precoat into the bottom left chamber. Allow it to flow through the middle chamber before filling it with the drug treatment.
To prepare the microfluidics chamber, sticky-Slide VI 0.4 was adhered to the glass coverslip. The 0.8-mm silicon tubing was connected to a programmable syringe pump. A rolling buffer containing 1% human serum albumin and 1 mM CaCl2 was added to the chamber. Then, the samples were incubated overnight at room temperature.
The somal and axonal chambers of a commercial microfluidics chamber have 120 capillaries of 150 mm length. Upon introduction of 30 mL of Matrigel (1:10), the compartments will separate. This phase separation creates a thirty-mL volume difference between the two. Using this method, it is possible to test benzene's toxicity.
A microfluidic chamber can be used to isolate cells from their bodies, monitor their migration, or analyze their functions. This device is easily manufactured using soft lithography and replica molding. Its simplicity and reproducibility make it a good choice for biology laboratories without clean-room facilities. Researchers have also developed multicompartment culture chambers to study neuronal processes. In addition to the microfluidic chamber, these devices are also useful in other applications, including cellular migration. View here to get more info about microfluidics chamber.
Human neural progenitor cells can be differentiated in the microfluidic device. The thin 3D matrix allows cleaner separation of axons from bulk neurons. The microfluidic chamber contains a membrane that regulates the production of amyloid b in FAD hNPCs, a cellular model of Alzheimer's disease. The protocol outlines how to isolate pure axons from bulk neurons. The biochemical profile is described in detail in Bhattacharyya et al.
For differentiation, resuspended cell pellets are placed in 30 uL of prechilled differentiation medium, containing 10% Matrigel. The conditioned media is then pipetted into the microfluidics chamber using prechilled pipettes. If bubbles appear in the CM, the samples should be pipetted again. If bubbles do appear, troubleshooting must be performed.
One example of the use of microfluidics in organ-on-a-chip technology is the development of the Organ-on-a-chip. Organ-on-a-chip devices are tiny, biocompatible chips that mimic the physiology of a cell. These devices have garnered tremendous attention in the drug-development field. Researchers can now test their drugs in realistic conditions, which would not have been possible before. To get more enlightened on the topic, click this link: xonamicrofluidics.com.
While the concept of lab-on-a-chip was first proposed in the 1970s, current microfluidic systems are still far from the vision. Microfluidic chips, for example, typically consist of channels carved into rigid substrates like glass or polymers, which limits their flexibility and functionality. Researchers have now discovered a way to dynamically configure the flow in these chips. This could revolutionize chemical and biological analysis. To get more enlightened on the topic, check out this related post: https://en.wikipedia.org/wiki/Microfluidic_cell_culture.